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KMID : 0545119920020010021
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Journal of Microbiology and Biotechnology
1992 Volume.2 No. 1 p.21 ~ p.25
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Production and Purification of Soluble Recombinant Human Lymphotoxin in Escherichia coli
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Choi, Yoon Ho
Na, Doe Sun/Pan, Jae gu/Park, Seung Kiel/Yoo, Hyang Sook/Kang, Kook Hee/Sung, Moon Hee
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Abstract
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Human lymphotoxin (LT) was produced in E. coli as a soluble protein. The level of recombinant human LT production was about 4% of the total souble proteins of E. coli extracts. Recominant human LT was purified to apparant homogeneity by a simple procedure utilizing FPLC on Mono Q and Mono S columns. The specific activity of the purified LT was 1.0×10 exp (7) units/㎎.
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